Welcome to the 2nd Necrotic Enteritis Conference.

We are pleased to welcome our national and international attendees made up of researchers, graduate students, pharmaceutical company professionals, industry professionals and stakeholders all interested in gaining knowledge about how to produce poultry with less/no antibiotics.  We know you will find much of interest in the program including the following:

Meeting Highlights

Guest Speakers

  • Dr. Rob Moore, Royal Melbourne Institute of Technology, Australia

  • Dr. Joan Smyth, University of Connecticut

  • Dr. Micheal Kogut, ARS-USDA

  • Dr. Filip Van Immerseel, Ghent University Belgium

  • Dr. Allen Byrd, Diamond V

  • Dr. Todd Applegate and Cristiano Bortoluzzi, University of Georgia

  • Dr. Marie Archambault, University of Montreal

Student Poster Session

Graduate students participating in the poster competition will present their posters on Wednesday, July 11 from 10:15 am to 11:15 am in the South Convention Lobby at the Sheraton.  The winner of the competition will be announced at the closing dinner, Thursday, July 12 at 6:00 pm in the Sheraton’s Grand Ballroom 1.

Closing Dinner

We have a fantastic dinner full of western USA flavors that is included in the attendee registration fee.  Meeting attendees who are interested in bringing a guest to July 12 dinner can purchase tickets for $30 at the Registration Desk on July 11 and until noon on July 12.

Thank you to our Sponsors

This conference would not be possible without the generous support of our sponsors.  Sponsors are recognized throughout the conference on signage, in the printed program and on posters and banners.  Many thanks to the individuals who worked to make the sponsorships possible.


The Registration Desk is located in the South Convention Lobby at the Denver Sheraton Hotel. The desk will be open as follows:

Tuesday, July 10           3:00 pm to 6:00 pm

Wednesday, July 11      7:00 am to 5:00 pm

Thursday, July 12          7:00 am to 4:30 pm

 Poster Information

1. Poster Dimension: 35.4 inches (90cm) wide by 46.5 inches (118cm) tall.

2. The poster area is the South Convention Lobby in the Denver Sheraton Hotel.  Posters must be up by 7:30 am on July 11.  Scheduled time to put posters on the boards is Tuesday, July 10 from 3:00 to 6:00 pm and Wednesday July 11 from 7:00 to 7:30 am.  The posters are numbered in the program and presenters should find the corresponding number on the poster boards to place their poster.  Please check with the registration desk to find the placement of any posters that are not listed in the program. Posters can be removed at 4:30 pm on July 12.

3. Student posters will be identified by a “Golden Chicken” sign.  Get the sign at the registration desk.  All student posters in the competition must have the sign with their poster.

4. Poster Session

Wednesday, July 11      10:15 to 11:15 am        Student Poster Competition and poster viewing.



July 11th 2018

7:00    Breakfast Courtesy of Zoetis, Grand Ballroom 1.

Sessions Held in Sheraton Denver Downtown, Grand Ballroom 2

8:00     Welcome         Charles Hofacre and Martine Boulianne

8:10     Opening note   John Smith, President AAAP

8:30     Guest speaker: ‘Genomics overview on Clostridium perfringens’, Rob Moore, Royal Melbourne Institute of Technology, Australia

Biology of Clostridium perfringens

9:15     Proteogenomic analysis of netB positive non-disease producing and netB positive necrotic enteritis producing Clostridium perfringens strains. Neha Mishra, University of Connecticut

9:30     Challenges with different Clostridium perfringens strains alter the expression of genes encoding proteins for apoptosis, mucin production and tight junction in broilers. Shu-Biao Wu, University of New England

9:45     The biofilm of Clostridium perfringens. Marie Archambault, University of Montreal.

10:00   Antimicrobial peptides secreted by Clostridium perfringens. Martine Boulianne, University de Montreal

10:15   Break and Posters session (for graduate students competition) in South Convention Lobby.

 Pathogenesis and virulence of Clostridium perfringens

11:15   Guest speaker: Understanding the pathogenesis of Clostridium perfringens. Joan Smyth. University of Connecticut

11:45   Comparison of pathogenicity of several Clostridium perfringens strains with Eimeria co-infection in inducing necrotic enteritis lesions in broiler chickens. Charles Li, ARS-USDA

Noon     Lunch courtesy of Evonik, Grand Ballroom 1. 

Pathologies caused by Clostridium perfringens

1:15     Pathology and diagnosis of necrotic enteritis of chickens. Francisco Uzal, California Animal Health and Food Safety Lab, UCDavis

1:30     Non-enteric lesions of necrotic enteritis in broiler chickens. H. L. Shivaprasad, University of California, Davis

Epidemiology of necrotic enteritis

1:45     Industry-wide survey of Clostridium perfringens Type A populations in US broilers and turkeys. Dan Karunakaran, Arm & Hammer

2:00     Necrotic enteritis and toxin analysis of Clostridium perfringens isolated from turkeys in California. H. L. Shivaprasad, University of California, Davis

2:15     Persistence of a Clostridium perfringens isolate in a poultry farm over a three-year period. Audrey Charlebois, University of Montreal

2:30     An epidemiological approach to studying the co-occurrence of Eimeria oocysts and Clostridium perfringens spores in litter from commercial broiler farms. Mark Jenkins, Animal Parasitic Diseases Laboratory, NEA, ARS, USDA

2:45     Beverage Break courtesy of Phibro, South Convention Lobby.

Immunity and Vaccination

3:15     Guest speaker: The role of gut immunity in the prevention of necrotic enteritis. Micheal Kogut, ARS-USDA

4:00     Does early exposure to Clostridium perfringens provide immunity against an oral Clostridium perfringens challenge at 17 days in a necrotic enteritis challenge model? Stephen Davis, Colorado Quality Research, Inc.

4:15     Attenuated Salmonella delivering three clostridial antigens to control necrotic enteritis. Kenneth Roland, Arizona State University

4:30     Plant-based vaccine to prevent necrotic enteritis in chickens. Amanda Tafoya, Arizona State University


July 12th 2018

7:00   Breakfast Courtesy of Zoetis, Grand Ballroom 1

Raising antibiotic free chickens

8:00     Guest speaker: Controlling the necrotic enteritis threat without antibiotics, a European experience’ Filip Van Immerseel, Ghent University, Belgium

8:45     Life Without Antibiotics: Field Lessons Learned the Hard Way. Timothy Cummings, Zoetis

Necrotic enteritis experimental models in research

9:15     Guest speaker: Necrotic enteritis: the pros and cons of experimental models. Allen Byrd, Diamond V

9:55     Effects of salmonella exposure times in a necrotic enteritis challenge model. Sam Hendrix, Colorado Quality Research

10:15   Beverage Break courtesy of Phibro, South Convention Lobby.

Control of necrotic enteritis with additives:

10:30   From in vitro to in vivo, an approach to assess organic acids and botanicals on necrotic enteritis. Ester Grilli, Dimevet, University of Bologna, Italy; Vetagro INC, Chicago, IL

11:00   Comparing a feed and drinking water application of butyric acid in broilers challenged with necrotic enteritis. Haci Bayir, University of Georiga

11:15   Probiotic, 1-monglycerides, and feed acidification improve performance and reduce necrotic enteritis when fed to broiler chickens challenged with Clostridium perfringens. Greg Mathis, Southern Poultry Research, Inc

11:30   Effect of replacing in-feed antibiotics with synergistic organic acids, with or without trace minerals and/or water acidification, on growth performance and health of broiler chickens under a Clostridium perfringens type A challenge. Yanming Han, Trouw Nutrition Agresearch

11:45   Effect of replacing in-feed antibiotics with synergistic organic acids on growth performance, health, carcass, and immune and oxidative status of broiler chickens under Clostridium perfringens type A challenge. Yanming Han, Trouw Nutrition R&D

Noon    Lunch courtesy of CHR Hansen Grand Ballroom 1. 

1:00     Evaluation of different doses of a novel Bacillus multi-strain DFM to control Clostridium perfringens induced necrotic enteritis (NE) in broiler chickens. Alfred Blanch, Chr. Hansen, A/S

1:15     Comparative efficacy of a novel multi-strain Bacillus-based DFM and each one of its single strains for the control of necrotic enteritis caused by Clostridium perfringens in broiler chickens. Dorthe Sandvang, Chr. Hansen, A/S

1:30     Evaluating the performance of Bacillus subtilis DSM 32315 under different necrotic enteritis models in broiler chickens. Adebayo Sokale, Evonik Corporation, Kennesaw, GA, USA

1:45     Effect of dietary supplementation of Bacillus subtilis DSM 32315 on the performance and intestinal microbiota of broiler chickens under Clostridium perfringens challenge. Cristiano Bortoluzzi, University of Georgia

2:00     Synbiotic (probiotic + prebiotic) as a potential alternative to ionophore to alleviate the effects of coccidiosis and necrotic enteritis in broiler chickens. G. Raj Murugesan, BIOMIN America Inc.

2:15     Use of Alquermold Natural as an effective manner to control Clostridium perfringens induced necrotic enteritis in poultry. Elvira Cano, Biovet, S.A.

2:30     Effects of valeric acid glycerides on performance, intestinal morphology, and protective effects during necrotic enteritis challenge. Jacob Lum, Perstorp Feed and Food

2:45     Necrotic enteritis prevention and control in broilers: The effects of an animal-use only antibiotic compared to shared-class antibiotics. Jaime Ruiz, Poultry Technical Consultant - Elanco Animal Health

3:00     Beverage Break courtesy of Phibro, South Convention Lobby.

3:15     Nutritional modifications to decrease risks of necrotic enteritis. Todd Applegate and Cristiano Bortoluzzi*, University of Georgia

3:45     Antimicrobial effect of selected yeast fractions on Clostridium perfringens. Giuseppina Avantaggiato, Institute of Sciences of Food Production - National Research Council of Italy (ISPA-CNR)

4:00     Efficacy of trace mineral-amino acid complexes in a necrotic enteritis challenge model. Charles Hofacre, Southern Poultry Research Group, Inc.

4:15     The use of in-feed mineral supplementation to control the impact of Clostridium perfringens on performance, lesion scores and necrotic enteritis associated mortality in various necrotic enteritis challenge models in broilers. Daniel Moore, Colorado Quality Research

4:30     Closing remarks

6:00 PM Banquet courtesy of Elanco, as well as best graduate student poster awards, Grand Ballroom 1.



Sheraton Denver Downtown, South Convention Lobby

Biology of Clostridium perfringens

Characterization of Clostridium perfringens netB+tpel+ Type A Strains Isolated from Necrotic Enteritis-Afflicted Broiler Chickens. Xianghe Yan, Beltsville Agricultural Research Center, ARS/USDA

Molecular characterization of type IV pilus operons in chicken Clostridium perfringens strains reveals a highly conserved organizational structure and a low level of gene sequence conservation. Marie-Lou Gaucher, University de Montreal

Pathogenesis and virulence of Clostridium perfringens

Viability of the Greater wax moth larvae (Galleria mellonella) as a model organism for the investigation of Clostridium perfringens associated disease (CPAD). Sammy Kay, Arden Biotechnology

Epidemiology of Necrotic Enteritis

Pulsed field gel electrophoresis and toxin type comparison of Clostridium perfringens isolates from birds with varied health statuses. Joseph Brown, Arden Biotechnology

Clostridium perfringens populations in the gastrointestinal tracts of healthy broilers in Asia. Evan Hutchison, Arm and Hamer

Prevalence of Clostridium perfringens isolates containing the netB gene in UK environmental sources. Joseph R. Edwards, Arden Biotechnology

Risk factors and their control

High body weight gain is a risk factor for the development and severity of necrotic enteritis lesions. Evelien Dierick, Ghent University

Interaction of necrotic enteritis severity and live salmonella vaccination on salmonella prevalence and load in broilers. Manuel Da Costa, Zoetis

Effect of selected Eimeria maxima strains on pathology and subsequent development of necrotic enteritis in a model system. Juan Latorre, University of Arkansas, Department of Poultry Science

Poultry feed linked with a clinical case of chicken necrotic enteritis in Quebec, Canada. A. Charlebois, E. Parent, D. Venne, M. Boulianne and M. Archambault, University of Montreal

Presence of NetB positive isolates of Clostridium perfringens in gizzards of broiler chickens at slaughter. A. Charlebois, A. Hayer, S. Lemire, M. Boulianne, and M. Archambault, University of Montreal

Diagnostic of Necrotic Enteritis

Optimization of an in vitro hemolysis assay for the detection of Clostridium perfringens NetB toxin in culture supernatant. Lore Van Damme, Ghent University

Evaluation of Media for the Enumeration of Clostridium perfringens from Poultry Feces. Martina Husta, Ghent University

Control of Necrotic Enteritis with additives

Evaluation of diet acidification with sodium bisulfate on broiler necrotic enteritis lesion scores, mortality, and performance in a Clostridium perfringens challenge model. Josh Payne, Jones-Hamilton Co.

Comparative efficacy of Alquermold Natural in a necrotic enteritis model as a treatment in drinking water and as a preventative in feed. C. Domenech, C.L. Hofacre, C.S. Hofacre, J. Pié, E. Cano, A. Tesouro

In vitro evaluation of the potential of lactic acid bacteria isolated from chicken ceca to inhibit Clostridium perfringens. Nuria Vieco-Saiz, Phileo Lesaffre Animal Care

Effects of NeutraPath™ with or without nicarbazin on the performance of broiler chickens during simulated field challenge conditions. Hongyu Xue, Amlan International

In vitro comparison of the growth inhibition concentration of two agents (essential oil blend and antibiotic) against 8 probiotics and Clostridium perfringens from monogastric gut flora. Isabelle Deuve Riou, Phytosynthese

Protective Effect for Broiler Chickens of Valerins and the Antibiotic BMD in Necrotic Enteritis Challenge Model. Charles Hofacre, Southern Poultry Research Group, Inc.

Evaluation of the in vitro and in vivo antibacterial effects of NeutraPath™. Dongping Wang, Amlan International




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